TLR1 Knockout cell line (THP-1)

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TLR1 Knockout cell line (THP-1) Order

Catalog#

YKO-H125

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TLR1 Knockout cell line (THP-1)

Cell Morphology

Monocyte,suspension

Passage ratio

1：2-1：4

Species

Human

Gene Name

TLR1

Gene ID

7096

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Accession

CVCL_E1DH(Cellosaurus)

Target region

Knockout region: Exon 4 (based on transcript TLR1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:THP-1			STR Info (Cell bank) Cell Line:THP-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13	13		13	13
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	11	13		11	13
D12S391	19	19
FGA	24	25
D2S1338	17	18
D21S11	30	31.2
D18S51	13	14
D8S1179	10	14
D3S1358	15	17
D6S1043	14	14
PENTAE	11	15
D19S433	12.2	13
PENTAD	10	12
D1S1656	16	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLR1 gene knockout TLR1 Knockout cell line (THP-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

TLR1 Knockout cell line (THP-1)

Catalog#

YKO-H125

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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TLR1 Knockout cell line (THP-1)

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